EMBL Conference From 3D light to 3D electron microscopy

Conference Overview
The event is jointly organised by EMBL, ZEISS, The Francis Crick Institute and the VIB Ghent.

Life happens in 3D – and understanding biological context in the three dimensions has been a major driver in the past decades in both light and electron microscopy. Today the trend towards 3D continues with large volume imaging and highest resolution with modern light and electron microscopy-based techniques now enabling previously impossible insights into functional and structural biology. Correlative microscopy can combine a large variety of different imaging techniques such as confocal microscopy, X-ray microscopy and different volume EM methods e.g. Array Tomography or Serial block face imaging. Comprehensive workflow solutions and automatisation have eased the path for the use of Correlative Microscopy and volumetric imaging.

An emerging challenge resulting from the improved connectivity between the individual imaging modalities is the handling of larger and thus more complex data. Workflow flexibility, file compatibility and big data handling are the new challenges to be tackled, not only from a hardware perspective but also for image processing and visualisation.

This meeting is centred on scientific sessions covering a broad range of correlative workflows, volume EM imaging, image processing techniques as well as segmentation and visualisation. The meeting will take place from 13 to 16 March 2022 – please save this date to your calendar! Don’t miss this exciting opportunity for exchange with researchers and industry to explore the new and emerging technologies and methods for correlative microscopy and volume EM in life sciences.

Due to the unpredictable COVID-19 situation, we are going to offer the 6th Joint Workshop and Symposium ‘From 3D Light to 3D Electron Microscopy’ as an onsite workshop at the EMBL in Heidelberg, Germany and for all of those who are not able to join in person we will broadcast parts of the event so you are able to join us remotely.

All participants are able to present their own scientific work in a poster presentation or an oral presentation. The organising committee will review abstracts for the oral presentations.

We look forward to meeting you either virtually or in person at the EMBL in Heidelberg!

Session Topics
vCLEM insights into biological structure and function
vEM and vCLEM on diverse sample types
New vCLEM workflows
New developments in vCLEM image processing
Workshops
Workshop 1 – FIB-SEM acquisition of single cells in a large sample guided by fluorescence
Workshop 2 – Breaking down the correlative workflow for Array Tomography: from section collection to image analysis
Workshop 3 – X-ray targeting of arabidopsis pollen tetrads for SBF-SEM
Workshop 4 – Correlative cryo workflow
Workshop 5 – ​Sample preparation for volume EM and vCLEM
Workshop 6 – Introducing the high-throughput serial section acquisition workflow with ZEISS MultiSEM
Panel Discussions
Panel Discussion 1 – vCLEM community around the globe
Panel Discussion 2 – Trends in vCLEM and vEM