- Conventional dendritic cells type 1 (cDC1s) specialize in antigen “cross-presentation”, a mechanism that involves the presentation of exogenous antigens on major histocompatibility complex class I (MHC-I) proteins. While this process is essential in triggering anti-viral and anti-tumor immunity, the molecular mechanisms that facilitate cargo export to the cytosol to promote MHC-I presentation after phagocytosis are largely unknown.
- Using correlative confocal and focused ion beam scanning electron microscopy, along with FRET and FRAP approaches, the authors show that phagosomal recruitment of the pore-forming protein apolipoprotein 7C (APOL7C) leads to phagosomal membrane rupture, leading to phagosomal content exit. Notably, APOL7C recruitment is dependent on NADPH oxidase-induced phagosomal membrane depolarization.
- These findings suggest APOL7C as a novel phagosomal effector required for efficient phagosomal cargo exit and subsequent antigen cross-presentation in cDC1s.
Read the Preprint:
MBoC's Preprint Highlights are commentaries written by Early-Career Editors on recent preprints of interest. They do not constitute peer review or imply publication of the original preprint by MBoC. For more information, visit https://www.molbiolcell.org/curation-tools.